Contact an Account ManagerSeparation of a 20mer oligonucleotide
Summary
Type: HPLC
Application #: 107420
Catalog#: 720252.40 - MN HPLC Column, Nucleosil C18 PPN, 100A, 5um, 4x250mm
Detection: UV, 290nm
Conditions: eluent A: 0.1M triethylammonium acetate pH 7.0 - acetonitrile (95:5, v/v), eluent B: 0.1M triethylammonium acetate pH 7.0 - acetonitrile (30:70, v/v), linear gradient from 15 to 40% B in 20min
flow rate 1ml/min
Authors: Macherey-Nagel
Source: LC department
Details
Legend: Separation of an oligonucleotide 20mer (courtesy of Dr. Essrich, Inst. Prof. Seelig, Karlsruhe, Germany). 1. 5'-hydroxy failure sequences, 2. benzamide, 3.
product peak (20mer), the following peaks a